A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers
نویسندگان
چکیده
We provide here a general introduction on chlorophyll (Chl) fluorescence, then we present our measurements fast (< 1 s) induction curves (the so-called OJIP transients) dark-adapted intact leaves of Arabidopsis thaliana, under five different light intensities [in the range ~ 500 to 3,000 µmol(photons) m‒2 s‒1] using two instruments: Handy PEA (Hansatech Instruments, UK; excitation light, 650 nm) and FluorPen (model FP-110; Photon Systems The Czech Republic; 470 nm). discuss observed differences in curves, as well Fo (F20μs, F50μs, or extrapolated Ft→0), FP peak), ratios FP/Fo, Fv (= ‒ Fo)/FP terms intensity absorptance (or absorbance) by leaves, other factors, data available literature. suggest that such be accompanied, future, parallel Chl fluorescence imaging, an area pioneered Hartmut K. Lichtenthaler.
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ژورنال
عنوان ژورنال: Photosynthetica
سال: 2021
ISSN: ['1573-9058', '0300-3604']
DOI: https://doi.org/10.32615/ps.2021.026